Author:
Sui Ming,Rong Jiachao,Zhang Yang,Qu Peng,Zhu Yusong
Abstract
Abstract
The gene of glycosyl hydrolase CeIA is derived from Bacillus licheniformis and the GenBank accession number is AAU40776.1. The sequence analysis of this gene was carried out by online software, and the celA gene was cloned from the genome of the strain by homologous amplification. The gene was ligated into the pMAL-c2x vector, transformed into E. coli Transetta for heterologous expression, and the catalytic properties of the recombinant enzyme CeIA were studied.