Determining the impacts of environmental parameters on model microbial community dynamics isolated from Rustumihia WWTP/Iraq

Author:

Noor T,Ralebitso-Senior T K,Sarker M,Wright D

Abstract

Abstract The composition of Rustumihia microbial community and their diversity with o-xylene-contaminants were investigated by applying molecular techniques, Polymerase chain reaction and denaturing gradient gel electrophoresis (PCR and DGGE) via investigating 16S rRNA gene fragments and understand the interrelationships between microbial community composition and structure for established microbial model community isolated from Rustumihia WWTP. To this end, that the established consortium could be used to assess the microbial response as defined by diversity and richness shifts, which are linked to changes in growth conditions. In this research paper a synthetic consortium was created by isolating indigenous microbial community members from the Rustumihia WWTP and subjecting consortium to different pH of (6.5, 7.0 and 7.5) and o-xylene concentrations of (0.5, 5 and 50 Mm) and temperatures (25°C, 35°C, 45°C and 55°C). The results of this study indicated that the high o-xylene concentration of 50 mM was tolerated and degraded effectively at 35°C and 55°C, and pH 6.5 (P < 0.001). Bacterial richness and diversity were recorded according to the Hill parameters of 0 D, 1 D and 2 D under each of the growth conditions, and then linked to the o-xylene degradation efficiency. At 35°C and pH 6.5, the consortium achieved high degradation percentage for each of 0.5, 5 and 50 mM of o-xylene with values 73.1%, 94.8% and 63.08%, respectively. The current study is the first of its kind in Iraq. It investigates the enrichment, isolation, and identification of a microbial community from the Rustumihia WWTP and determines the efficiency of the isolates to tolerate and degrade o-xylene, highlighting their sole source of hydrocarbon. This research underscores the usefulness of molecular techniques for both diversity and richness to understand the ecological impact of o-xylene as a contaminant and to identify potential molecular techniques for detection of gene that is responsible for o-xylene degradation.

Publisher

IOP Publishing

Subject

General Medicine

Reference91 articles.

1. Fingerprinting petroleum products: unleaded gasolines;Potter;Petroleum Contaminated Soils,1990

2. Microbial Biodegradation: From Omics to Function and Application

3. Gene expression during BTEX biodegradation by a microbial consortium acclimatized to unleaded gasoline and a Pseudomonas putida strain (HM346961) isolated from it;Chavez;Polish Journal of Microbiology,2017

4. Biodegradation of aromatic compounds by Escherichia coli;Diaz;Microbiology and Molecular Biology Reviews,2001

5. The influence of cell immobilization by biofilm forming on the biodegradation capabilities of bacterial consortia;Lawniczak;World Journal of Microbiology and Biotechnology,2011

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3