Optimization of self nanoemulsifying drug delivery system with ethanolic extract of ginger (zingiber officinale) and eel bone oil (anguilla spp.) extracted by maceration as carrier

Abstract

Abstract SNEDDS is a drug delivery method by mixing oil, surfactant, cosurfactant and drug that can form spontaneous nanoemulsion oil in water in the digestive tract and produce nanometer-sized droplet. Aim of this study was to get optimation for SNEDDS (Self Nanoemulsifying Drug Delivery System) with ethanolic extract of ginger, eel fish bone oil extracted by maceration as carrier, tween 80 as surfactant and PEG 400 as cosurfactant that applicable as a nanoemulsion formula. Optimation formula was measured based on transmittance and emulsification time of mixture of eel fish oil, ginger extract, tween 80, and PEG 400 into 8 different compositions. The Transmittance values were measured using UV/VIS Spectrophotometric at 650 nm and emulsification times were measured using stopwatch by diluting 1 mL of SNEDDS into AGF (Artificial Gastric Fluid), aquadest, and aquabidest. The optimum SNEDDS formula was determined based on the optimization results using Design Expert 7 program with the independent variable was composition of tween 80 and composition of PEG 400 and the dependent variable was transmittance and emulsification time in AGF, aquadest, and aquabidest. Result of Design Expert 7 program optimization formula showed that SNEDDS of ginger extract will be optimized in 4.2 g of tween 80 as surfactant; 0.25 g of PEG 400 as a cosurfactant; 500 mg of ethanolic ginger extract and 0.55 g of maceration extract of eel fish bone oil with transmittance value was 92 ± 0.034 %; emulsification time in AGF was 10 second, in Aquadest was 10 second, and Aquabidest was 9 second. The result of Analysis of Variance indicated not significant differences between treatment groups and optimization by design expert 7 program ((P = 0.1359 (P>0.05)).