KCC2 overexpressed exosomes meditated spinal cord injury recovery in mice

Abstract

Abstract Exosomes show great potential in treating diseases of the central nervous system including spinal cord injury (SCI), still better engineered exosomes have more advantages. In this study, we purified exosomes from K+–Cl− co-transporter (KCC2) overexpressed bone marrow mesenchymal stem cells (ExoKCC2), to investigate the effect of ExoKCC2 on neural differentiation in vitro and the repairing function of ExoKCC2 in SCI mice in vivo. Compared to bone marrow mesenchymal stem cells (BMSC)-derived exosomes (Exo), ExoKCC2 could better promote neural stem cell differentiated into neurons, ameliorate the function recovery of SCI mice, and accelerate the neural regeneration at the lesion site. Altogether, engineered ExoKCC2 may prove to be an advantageous strategy for SCI treatment.