PyEcoLib: a python library for simulating stochastic cell size dynamics

Abstract

Abstract Recently, there has been an increasing need for tools to simulate cell size regulation due to important applications in cell proliferation and gene expression. However, implementing the simulation usually presents some difficulties, as the division has a cycle-dependent occurrence rate. In this article, we gather a recent theoretical framework in PyEcoLib, a python-based library to simulate the stochastic dynamics of the size of bacterial cells. This library can simulate cell size trajectories with an arbitrarily small sampling period. In addition, this simulator can include stochastic variables, such as the cell size at the beginning of the experiment, the cycle duration timing, the growth rate, and the splitting position. Furthermore, from a population perspective, the user can choose between tracking a single lineage or all cells in a colony. They can also simulate the most common division strategies (adder, timer, and sizer) using the division rate formalism and numerical methods. As an example of PyecoLib applications, we explain how to couple size dynamics with gene expression predicting, from simulations, how the noise in protein levels increases by increasing the noise in division timing, the noise in growth rate and the noise in cell splitting position. The simplicity of this library and its transparency about the underlying theoretical framework yield the inclusion of cell size stochasticity in complex models of gene expression.