Comparative evaluation of wavelength-scanning Otto and Kretschmann configurations of SPR biosensors for low analyte concentration measurement

Abstract

Abstract The growing demand for compound characterization has stimulated research, particularly in surface plasmon resonance technology. This technique monitors changes in the light-reflecting properties of a sample medium in close contact and in interaction with a plasmonic surface (typically a metal such as gold) due to shifts in the fundamental plasmon resonance of the surface. The Otto and Kretschmann configurations are commonly used in this method. When an analyte is expensive, scarce, or hazardous, it is advantageous to reduce the sample required for testing, making optimization of sample use interesting. This challenge requires trade-offs between sensitivity and LoD. This work compares two sensors in the indicated configurations designed for minimal analyte requirement (in this case, Ag nanoparticle suspensions) using the wavelength scanning technique. The results show that the Kretschmann configuration is the most efficient for characterizing nanoparticle suspensions due to its construction characteristics, ease of use, and the characteristics of the obtained response. The final arrangement is a quasi-point sensor that only requires 6μL of analyte and has a sensitivity of 4.17x10−4 RIU/λ (RIU is the refractive index unit). This study contributes to the exploration of advantages and limitations in the design and operation of SPR sensors. The work also underlies the need for future research to enhance the selectivity and versatility of these devices.