Detection the effect of culture medium supplied with fetal bovine serum on the proliferation of gonad cell cultured for rats Rattus norvegicus

Abstract

Abstract This paper aimed to evaluate the role of fetal bovine serum (FBS) on the proliferation cultured gonads cells of white male rats by detection the impact of suitable concentration of (FBS) for gonads cell cultured in vitro. To perform the experiment the gonads of the rats were dissection out to obtain the gonad cells, then the obtained gonad cells were cultured in minimal essential medium (MEM medium) added to it different concentration of fetal bovine serum (10%,20%), the cells were cultured in (MEM medium) supported with (FBS) showed higher proliferation ratio (91.66±0.33) compare with the proliferation ratio at minimal essential medium (MEM medium) 76.66±0.33 and control (4.66±0.33), in the same direction the proliferation ration in medium supported with (20% FBS) 34±0 exceed the proliferation ratio in the medium supported with(10% FBS) 18±0 after two weeks from the culturing of gonad cells. FBS had positive role to support the proliferation of gonad cells for white rats male.