Studying Hypoglycemic Activity of Cuscuta chinesis Lam. on Type 1 Diabetes Mellitus in White Male Rats

Abstract

Abstract This study was conducted to evaluate the Hypoglycemic activity of Methanolic-Watery Extract of Cuscuta chinesis Lam. plant by screening about the presence of some antidiabetic and antioxidant phytochemical compound by HPLC technique in addition to estimate the effect of this extract on fasting blood glucose (FBG) of type 1 diabetic rats.Four runs of Preparative reversed-phase HPLC was performed using column C18-ODS (25cm X 4.6 mm X 5 μm) as astationary phase, While The mobile phase determined based on the type of detected phytochemical, which were (Berberine, Kampferol, quercetin and β-carotene). And the experiment demand devidenig 54 Rattus rattus male rats weighting (180 -200 g) into 3 groups: A normal control daily administrated with Dw, B Diabetic control daily administrated with Dw and C diabetic group daily administrated with 400 mg/Kg body weight of C. chinesis Lam. methanolic extract, each group consisted of 18 rats, and further divided into (3) sub- groups 1, 2 and 3. According to the period of administration 30, 60 and 90 days respectively. The results showed the precence of (Berberine, Kampferol, quercetin and β- carotene) in the following respectively in concentration (246, 13.3, 320 and17977) ppm. in addition to that the results showed significance decrease in FBG of group C as the period of extract administration increase in compare with group B, which had FBG ( 125, 90 and 59) mg/dl after ( 30, 60, 90)days of extract administration respectively. So we concluded that administrated diabetic rats with 400 mg/Kg body weight of C. chinesis Lam. extract for two month retained the FBG to the normal level.