Comparative Study morphological and Molecular for infected Rodent with two cestoda Hymenolepis nana & H. diminuta In Al-Diwaniyah City, South Of Iraq

Abstract

Abstract The study aimed at comparing infection of Hymenolepis nana with that of H. diminuta during the study period from November 2016 to August 2017, A total of 220 rodents were identified including Mus musculus(89), Rattus norvegicus(37), R. rattus(48) & Swiss albino(46). Under anesthesia by chloroform, specimen were dissected, seeking parasitic helminths within digesting bowels. It was found that Mus musculus, Rattus rattus, Rattus norvegicus and Swiss Albino were infected by H. nana (4.4%, 4.1%, 8.1%, 13%) respectively, comparison with Mus musculus, Rattus rattus and Rattus norvegicus were infected by H. diminuta (31.4%, 39.5%, 13.5%) respectively. Diagnosis is confirmed by using PCR technique for H. nana using ITS1 gene, and for H. diminuta using ITS2 gene. In addition to diagnosis is confirmed by using Gen Sequencer technique, through out the analyzing of phylogenetic tree leading to learn the extent of convergence between the local specimens and the global ones, the diagnosis of these helminthes by using this technique is considered the first in Iraq.