Investigation of toxoplasmosis in sheep in Al-Diwaniya city by using modern technique

Abstract

Abstract Toxoplasma gondii, it’s not limited to the disease in sheep on the abortions and loss of births only, but beyond the transfer of infection to humans through consumption of sheep not cooked well meat infected with parasite, its considered one of the important sources of infection human with this disease, T. gondii were diagnosed in 80 blood samples of sheep at Al- Diwaniya city by using latex test and molecular diagnosis with Polymerase Chain Reaction depending of presence B1 gene of T. gondii, the results showed that antibodies were detected in 13 samples out of 80 (16.25%). of sheep since significant(P⩽ 0.05) in highest titration indicated at 1/80 (38.17%), while the lowest titer indicated at 1/40 (10.09), the results of Polymerase chain reaction for detection of B1 gene in blood of sheep showed that (7 of 80)(8.75%) of positive samples from the sheep and (73 of 80) negative samples. It had been concluded that sheep were found infected with T. gondii with possible transmission this disease to human and extends to public health through the consumption of infected meat that facilitates transmission of the disease to humans.