Gene expression of some cytokines in patients with Cutaneous leishmaniasis in Al-Diwanyah province

Abstract

Abstract The current study was conducted in the Department of Biology - Faculty of Education for Girls - University of Kufa for the period from March 2018 to August 2018, which aims to detect the genetic expression of the mRNA of some of the cytokines in the peripheral blood of patients with cutaneous leishmaniasis using RT-qPCR technique. The results showed that the relative quantification of gene expression of mRNA for IFN-γ, IL-4, IL-10 andTNF-α in the blood of patients with cutaneous leishmaniasis compared to control. The highest amount was TNF-α, followed byIFN-γ, IL-10 and IL-4, which was 15.9±4.9, 10.5±4.6, 7.6±3.7 and 3.7±1.1 respectively, compared with control of 2.3±1.2, 2.6±1.2, 2.6±1.2 and 1.9±1.3 respectively, with a statistically significant difference at P <0.05. The correlation results showed that all cytokines (IFN-γ, IL-4, IL-10, TNF-α) were significantly correlated with each other in terms of gene expression and the association was strong betweenIFN-γ and IL-10 (r = 0.88), followed by IFN-γand TNF-α (r = 0.75) and IL-10 and TNF-α, (r = 0.63) and then IL-4 and IL-10 (r = 0.54).The conclusion from this study that a cellular immune response is achieved by increasing the genetic expression of cytokines, IFN-γ, IL-4, IL-10 and TNF-α in the peripheral blood of patients with cutaneous leishmaniasis and all these cytokines are positively correlated with each other.