Detection OF β- lactamase genes in clinical Klebsiella pneumonia strains isolated from hilla hospitals

Abstract

Abstract This study aimed for isolation and identification of Klebsiella pneumonia, from clinical sample and from different hospitals in Hilla city. during the period from April to October 2017. 122/1056 (12%) isolates were belonged to Klebsiella pneumonia were collected. Growth on blood agar, MacConkey agar and Eosin methylene blue agar was identified by cultural, morphological and biochemical tests and confirmed by VITEK 2 system. The results showed that all the tested isolates were resistant to Ampicillin and Amoxicillin 122(100%), while 119(98%) for penicillin, whereas 100(82%) for piperacillin. Resistance to other drug classes varied among the isolates, a higher resistance was also detected with 95(78 %) to cefotaxime 99(81%) ceftazidime, 94(77%) to ceftriaxone and 92(75%) to ceftriaxone, The resistance to quinolones, nalidixic acid, ciprofloxacin and levofloxacin was detected 57(47%), 39(32%), 35(29%), respectively.majority of isolates 42 (34.4%) were obtained from sputum samples this result, 34 (27.8%) from urine, 17 (14 %) from stool, 15 (12.2 %) from burn, 8 ( 6.6 %) from vagina, 4 (3.3%) from wound, 1 (0.8%) from blood, 1 (0.8%) from ear and 0 (0 %) from both eye and throat. High prevalence rate for MDR, XDR and PDR in Merjan Teaching Hospital. The proportion of the patients from Merjan Teaching Hospital 27 (35.5%), 16 (44.4%) and 1 (50%) respectively. Detection of β - lactamase genes (blaCTX-M-9, blaCTX-M-10, and blaNDM-1) was performed by the conventional PCR technique. The results revealed that 36 isolates analyzed, blaCTX-M-9 12 (33.3%), blaCTX-M-10 14 (38.9%), and blaNDM-1 (0%).