The effect of siam orange juice (Citrus nobilis Lour.) in extender on Garut Ram (Ovis aries L.) spermatozoa quality post-cryopreservation

Abstract

Abstract The research aimed to find out the effect of Siam Orange juice in extender on Garut Ram spermatozoa quality 24 hours post-cryopreservation. Semen was collected from five rams once a week using an artificial vagina. Semen sample was diluted in Tris-egg yolk-based extender containing 0 % (KK), 5 % (KP 1), 10 % (KP 2), 15 % (KP 3) and 20 % (KP 4) Siam orange juice. Semen was packed in 0.25 ml straw with a final concentration of 50x106 spermatozoa/mL. Sperm was equilibrated at 5° C for two hours then frozen and stored in liquid nitrogen (LN2) tube. Sperm parameters, including motility, viability, membrane integrity, acrosome integrity, and abnormality, were assessed. One-way ANOVA test results showed significant differences between treatment groups on the percentage of motility, viability, and membrane integrity (p <0.05). Duncan test results indicated that KP 2 had significant differences in all treatment groups. Therefore, orange juice at 10 % concentration (KP 2) was the best concentration that was able to minimize the reduction of ram spermatozoa quality post-cryopreservation based on motility [(58.68 ± 0.68) %], viability [(59.73 ± 6.47) %], and membrane integrity [(54.87 ± 5.58) %].