Detection of Toxoplasma gondii genotypes in abortion women by RFLP-PCR in Al-Najaf Al-Ashraf province

Abstract

Abstract The study conducted on 400 aborted women and 30 healthy women as control groups. All these cases were defined as suspected with Toxoplasma gondii and examined by measuring IgG & IgM serum level when attended to Public Health Laboratory, in An-Najaf province from November 2015 to May 2016.seventy eight out of 400 (19.5 %) women were found infected with T. gondii by this test, only 23 positive samples were used in polymerase chain reaction – restriction fragment length polymorphism for detection genotypes of T. gondii strains By use three restriction enzymes MseI to nested PCR products GRA6 and BsiE1 & TaqI to nested PCR BTUB products, to detected strain types of Toxoplasma gondii. The results are : 13 of type I (60%), 6 of type II (25 %), and 4 of type III (15%). This can provide possible additional studies of high importance, as they will provide better understanding of the different strains of parasite genotypes is present in samples and how different genotypes are circulating nature. All the evidence provided might be helpful for showing the association between strains type of T. gondii and toxoplasmosis in infected women and aid in providing preventive actions.