Effect of bee venom on some blood and biochemical parameters in formaldehyde induced arthritis male rats in comparison with prednisolone drug

Abstract

Abstract Background Bee venom(BV) has been used to treat and reduce chronic inflammatory diseases such as rheumatoid arthritis(RA), pain, skin diseases and to treat cancerous tumors. The aim of this study was to investigate the effects of bee venom on some blood and biochemical parameters in male albino rats with Arthritis and compare it with prednisolone drug. Materials & methods: (42) mature male albino rats (135-150) gm, divided into (7) groups, (6) male rats for each group. The experiment was continued for (14) days and included: the first group (negative control) was injected by (0.1ml/animal) from physiological normal saline (0.9% Nacl),the second group(Arthritis group) was injected by (0.1ml/animal) formaldehyde, the third group (normal group) was injected by bee venom(i.p) (1mg / kg of B.W), the fourth group (normal group) was treated orally with prednisolone (5mg / kg of B.W), the fifth group (Arthritis group) was injected by bee venom(i.p) (1mg / kg of B.W), the sixth group (Arthritis group) was treated orally by prednisolone (5 mg / kg of B.W) and seventh group (Arthritis group) was injected by bee venom (1 mg/ kg of B.W) and treated orally with prednisolone (5mg / kg of B.W). Results: Our results showed that 2nd group (Arthritis) significantly increased (p≤ 0.05) in the number of WBCs and platelets compared with control group, while the 3rd and 4th groups showed a significant decrease in WBCs and PLT compared with 2nd group. The 5th, 6th and 7th groups showed a significant decrease (P≤0.05) in the number of WBCs and platelets compared with 2nd group. On the other hand, the present study demonstrated a significant decrease (p≤ 0.05) in RBCs, hemoglobin (Hb) and hematocrit (HCT) in the 2nd group (Arthritis group) compared with control group, while the 3rd and 4th groups showed a significant increase in RBCs, Hb and HCT compared with 2nd group. The 5th, 6th and 7th groups showed increase (p≤ 0.05) in RBCs, Hb and HCT compared with 2nd group. The results showed also, a significant increase in levels of cholesterol, triglycerides, LDL and a significant decrease in HDL level in 2nd group compared with control group, while 3rd,4th, 5th, 6th and 7th groups showed a significant decrease (P≤0.05) in cholesterol, triglycerides, LDL and a significant increase in HDL level compared with 2nd group. Conclusion: We conclude from the present study that bee venom attenuates development of Arthritis by improving some Blood and Biochemical parameters.