Simple Amperometric Biosensor for Sucrose Concentration Measurement Based on Principal Component Analysis

Abstract

Abstract Sucrose is a type of sugar that is widely used in various types of foods and beverages. In Indonesia, sucrose consumption reaches 2.8 million tons on average per year. Effects of consuming too much sucrose can increase the risk of various diseases such as diabetes, dental caries and obesity. The level of maximum amount of sucrose that is safe for the body equal to 10% of the total energy or the equivalent of 50 g/person/day, so that the required detection system and the identification of the sucrose concentration. In this work, the identification process was carried out using an amperometric biosensor based on the yeast Saccharomyces cerevisiae as a bioreceptor. Measurements were made by immobilizing yeast cells and analyte samples into the biosensor electrodes and observed based on cellular respiration activity which was expressed as a parameter of dissolved oxygen (DO). The biosensor response is generated in the form of an output potential value, then processed using principal component analysis (PCA) to produce a sucrose concentration classification point with a percentage of variance of the two main components of 98.77% which states that the sensor is able to identify sucrose concentrations.