Green Synthesis, Characterization and Biological Activity of Silver Nanoparticles Using Ruta Leaf Extract

Abstract

Abstract In this paper, green synthesis of AgNPs is carried out by means of an environmentally friendly, cost-effective and safe technology using Ruta leaf extract as a reducing agent for silver. Furthermore, biological activity of produced AgNPs was examined against different microorganism with multiple resistances to antibiotics, which isolated from different disease states. The produced AgNPs were characterized by UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Field Emission Scanning electron microscopy (FESEM) and Dynamic light scattering (DLS). The biosynthesized silver nanoparticles by Ruta leaf extract were confirmed by their change of color from colorless to yellow and lastly to reddish brown. UV-visible spectrophotometric analysis of the AgNPs synthesized showed characteristic surface plasmon absorption peak at 415 nm. Where the X-ray diffraction showed that, the nanoparticles were crystalline in nature with a face-centered cubic structure (FCC). Field Emission scanning electron microscopy (FESEM) micrograph showed the creation of spherical nanoparticles with size from 30 to 50 nm in diameter. The element silver of the sample was obtained from the Energy Dispersive Analysis of X-ray (EDX). The stability and distribution size were detected using zeta potential and DLS analysis.