Effect of ultrasonic technology on cellulase enzyme activity produced by local bacterial isolate

Abstract

Abstract The research aimed to obtain new bacterial isolate producing cellulase enzyme, and ultrasonic effect on enzyme activity. Three bacterial isolates were isolated from different agricultural wastes samples. Their effectiveness in cellulolytic was detected by measurement of the clear zone diameter around bacterial growth. The enzyme was extracted after the growtht of local bacterial isolate which has highest enzyme activity. The enzyme was exposed to ultrasound intensity of 40 KH, low, medium and high power, and exposure time (10, 20, 30, 40, 50, and 60) min, enzymatic activity was measured after each treatment compared with control. It was found at low level that an increase in the cellulase activity with increased ultrasonic exposure time, the higher activity at 60(min). The effect of ultrasonic treatment at mid-level, showed higher activity at 20 (min), while at high level the activity decrease with exposure time when compared with control. Protein concentration was estimated, there was no significant difference on protein content after ultrasonic treatment in samples at (10, 20, 30, 40) min while decrease in protein content in samples at (50, 60) min, compared with with control.