Hatchability genotype of Central Javanese local ducks based on ovomucoid gene

Abstract

Abstract Ovomucoid genes are related to hatchability of duck eggs. The study would analyze the genotype of local duck hatchability of Central Java based on ovomucoid genes by the PCR-RFLP. This research is an exploratory study using 35 samples of local Central Java duck. Central Java local duck hatching genotypes were detected by ovomucoid genes fragment cutting as a result of PCR amplification using ApoI restriction enzymes. DNA isolated from duck feathers using the gSYNC ™ DNA Extraction Kit. Amplification ovomucoid gene was done by PCR using TovF2 and TovR2 primers. The results showed that entire samples were genotyped +/+ (high hatchability), because they were not cut off by the ApoI restriction enzyme. The results of nucleotide sequences showed that Central Java local ducks had no cutting site for the ApoI enzyme (5’-AAATTC-3’), because there were mutations in the nucleotides to A577G and A579C and no nucleotide deletions to 576-578. It was concluded that based on ovomucoid genes, all Central Java local duck samples were genotypes with high hatchability (genotype +/+). This previous research, strengthens previous argument that local Central Java ducks need to be maintained or preserved as laying ducks with high hatchability.