Sugarcane of Rapid Multiplication by Callogenesis

Abstract

Abstract The results showed that shoot apical meristem of sugarcane has the ability to stimulate and induction to callus in vitro by plant tissue culture. The results showed 2,4-D regulator 1.5 mg/L was more potent in the induction of the callus and callus subsequent growth. The effected interactions of auxin and cytokinin regulators were not significant with respect to the formation of callus. The best regeneration response was achieved by using growth regulators. In Co8371, best induction of the shoot response was found on MS medium with concentration of 1.0 mg/L BAP compared to Co85004. MS medium with the using of 0.5 mg/L BAP and 0.25 mg/L Kinetin regulator exhibited best organogenesis responding.