Hepatoprotactive Effect of Lallemantia royleana Seeds Extract Against the Toxicity of Rifadin Through Reduction BAX Expression in Liver Tissue of Albino Mice

Abstract

Abstract This study was aimed to investigate the role of crud alcoholic extract of Lallemantia royleana seeds thruogh reduction the hepatotoxicity of rifadin drug in liver tissue. The animal (40 mice) were divided into four groups the first group treated with normal saline (0.9%) for 28 days as acontrol, the second group treated with rifadin (1.5 mg/kg/day) for 28 days, third group treated with acoholic extract of Lallemantia royleana seeds (1% w/v) for 28 days, while the forth group treated with alcoholic extract of seeds alone for 5 days and with alcoholic extract and rifadin for 28 days, so the total period of this group is 33 days. The liver specimens were collected and processed with immunohistochemical staining kit to detect Bax protein in hepatocytes. The microscopic examination results of liver slides coloured with imununohistochemical staining technique in animal experimental groups showed increased in the number of cells with positive immunoreaction for Bax in animal group treated with rifadin drug comparing with other experimental groups (seeds extract and drug group, seeds extract group, control group)which showed significant decreasing in the number of cells with positive immunoreaction for Bax.