Characterization of indicator electrodes using the potentiometric biosensor method as urea sensor with XRD, SEM-EDS, UV-Vis and FTIR

Abstract

Abstract The purpose of this study is the characteristics of the indicator electrode which has been coated with a membrane with XRD, SEM-EDS, UV-Vis and FTIR. Research has been carried out using XRD, SEM-EDS, UV-Vis and FTIR tests on immobilized indicator electrodes 0.0350 g PVA-Enzyme coated with PVC-KTpClPB 0.0120 g and 0.0500 g. The method used was the biosensor potentiometric method with the urease enzyme immobilization technique in PVA coated with PVC-KTpClPB. To see the difference in the absorbance spectrum pattern of each PVA-Enzyme composition coated with PVC-KTpClPB against wavelength with UV-Vis, PVA-Enzim solution as a reference and PVC-KTpClPB solution as the first analysis 0.0120 g and as the second analysis 0,0500 g. To see the PVA-enzyme membrane coated with PVC-KTpClPB in the two compositions above was characterized by FTIR and SEM-EDS. To see the indicator electrode that had been immobilized by PVA-enzyme coated with PVC-KTpClPB in the two compositions above, it was characterized by XRD. It was concluded that the best results were found on the immobilization indicator electrode of 0.0350 g PVA-Enzyme coated with 0.0350 g PVC-0.0500 g KTpClPB.