Modern microbiological methods of phytosanitary monitoring

Abstract

Abstract In this work, an express method is proposed, based on the registration of changes in the impedance of the nutrient medium, which occurs under the influence of the processes of growth and vital activity of microorganisms in the sample under study. The research was carried out on the analyzer “BioTrack 4250”. This device is highly productive, as it examined 21 samples simultaneously for several hours. For testing the indirect impedance technique, fodder, medicinal and weed plants were selected as objects of study. The sanitary condition of the samples was assessed by the total mesophyll aerobic and optional-anaerobic microorganisms (QMA&OAMO), sanitary-indicative bacteria of the E. coli group, and the number of yeast fungi. It was proved that plants collected in the central part of the city of Tyumen with a high population density had high QMA&OAMO indices and high indicators of contamination with Candida sp. On the contrary, fodder plants growing in the Tyumen region were characterized by high indices of sanitary-indicative microorganisms, which indicated an increased risk of feed contamination. Thus, in contrast to the classical methods, the proposed method allows obtaining objective results, shortening the research time, reducing labor costs, and significantly reducing the cost of analysis.