Affinity materials for Phosphorylated and Glycosylated Proteins/Peptides enrichment

Abstract

Abstract Two typical types of protein post-translational modifications (PTM), protein/peptides phosphorylation and glycosylation play important roles in numerous biochemical processes including signal transduction, cell division, protein secretion and phagocytic vesicles trafficking. Mass spectrometry (MS) is one of the most widely used tools for PTM analysis. Although mass spectrometry is one of the most powerful analysis tool, it is always interfered by impurities and almost impossible to analyze phosphorylated and glycosylated proteins/peptides directly from complicated bio-sample because of low abundance of target proteins/peptides and interference of non-target substances. Thus, enrichment of phosphorylated and glycosylated proteins/peptides is an essential procedure before MS analysis to ensure the effectiveness of MS detection. In this review, recent progress in phosphorylated and glycosylated proteins/peptides enrichment strategies is summarized.