Effect of LED Wavelengths and Light-Dark Cycle on Photosynthetic Production of Chlorella Kessleri for Algae-Based Biosensor Optimization

Abstract

Abstract Observation of cell culture with a micro sensor system for biomedical application in measuring chemical, biological and physical parameters are widely used to evaluate cell metabolism respiration. Cellular respiration depicts the energy formation and molecules in the form of dissolved oxygen (pO2) level needed in the photosynthesis process. This paper presents the observation of pO2 level produced from the algae chlorella kessleri influenced by LED light source at wavelength of 480 nm (blue) and 650 nm (red). It aims to show the relationship between photosynthesis production activities to the signals produced by each condition for biosensor optimization. Artificial light settings consist of light-dark cycle to simulate a day light period in producing chlorophyll and night period as a time of relaxation. Sample of 150μL chlorella kessleri with density 1.4·106 cell/ml is immobilized in biochip-C and stimulate with two difference wavelength light sources for 30 minutes period ON/OFF. The result shows that the red light has 18% pO2 level higher than blue light since the algae is sensitive to the red light and absorbed more energy to produce chlorophyll.