Spectrophotometric Determination Of Glutathione By Iodate / Iodide

Abstract

Abstract spectrophotometric method was applied for determination of GSH by reaction iodate and iodide at λmax=350 nm with high efficiency. The calibration curve was fabricated with linear range (1-35)ppm. The linearity (R2) value was 0.9980. The limit of detection value was 0.0002 ppm. The limit of quantification value was 0.0004 ppm. The effect of interferences on the determination of GSH was studed under optimum conditions. The interferences with 10 twice of concentration (100) ppm were added individually to the sample solution containing 10 ppm of GSH. It was noticed that the interfering components not effected on determination of GSH. Composition of complex (GSH/KIO3) was determined by mole ratio method which appear ratio about (1:1). Determination of GSH was applied on standard solutions, which gave high precision and accuracy with recovery in well worthily.