Comparison of Cm ACS-7 Gene Expression in Melon (Cucumis melo L.) Flowers

Abstract

Abstract Melon (Cucumis melo L.) is a member of the Cucurbitaceae family spread throughout the world. Melons have very varied sexual types, which can be important information in genetic analysis and breeding. Sex type jumps from genetic factors, environmental factors, and environmental conditions. The aim of this study was the expression of CmACS-7 gene expression in melon flowers in various sexual types. This study used melons with andromonoecious sexual types (Melodi Gama 1 and Melodi Gama 3 cultivars) and monoecious (PI 371795 and Bartek). This study uses quantitative PCR analysis using real-time PCR with relative quantitative methods. RNA was isolated and then synthesized into cDNA, then amplified using specific primers. The number of cDNA specific genes was analyzed using Real-time PCR to calculate the level of gene expression. The results showed that the CmACS-7 gene was expressed more in female and hermaphrodite flowers than in male flowers. In male flowers with andromonoecious sexual type, the CmACS-7 gene is expressed more than in male flowers with monoecious sexual type. This research is expected to be important information in breeding melon plants.