Acquisition and segmentation images algorithm for the temporary study of the dynamic speckle phenomenon

Abstract

Abstract Dynamic speckle is an interferometric phenomenon, which has been considered a sensitive way to monitor the weak changes in biological samples, and therefore it is a reliable tool that can be applied in many areas, from medicine to farming. Its use demanded the appearance of a series of methods to illuminate, process the images and provide their analysis. For this reason, for its implementation it requires systems of acquisition of images and algorithms of detection or identification of the biological material of the rest of the whole of the image. This work proposes an algorithm that allows the acquisition and segmentation of biological samples of plant origin. The algorithm developed requires the CMOS camera of a cell phone for the acquisition and transmission of the images of size 720x480 pixels, a computer for the management, reception and processing of the same, a wireless local area network, a He-Ne laser 633 nm with 10 mW of power as a coherent light source, an optical diffuser and an aluminum surface for the placement of biological samples. The study showed satisfactory results to acquire images and store them, allowing their subsequent segmentation.