Application of a microspectral analysis for evaluation of the morphofunctional status of immunocompetent cells in cattle with retroviral diseases

Abstract

Abstract Microspectral analysis is a highly informative way to study the morphofunctional status of cells, including immunocompetent ones. Purpose of the study was an application it for studding of lymphocytes in cattle with enzootic leukemia and viral immunodeficiency. It is important for diagnostics and differential diagnostics of diseases, as well as for analyzing of animal homeostasis and making a prognosis of the disease. Spectral analysis of stained with a Leukodif 200 kit agranulocytes was carried out using the universal color analyzer microscope-spectrophotometer LOMO MSFU-K. The value of the light absorption intensity was measured in the spectrum from 300 to 700 nm. The results of microspectral analysis showed that for cows’ lymphocytes with combined pathology (BLV/BIV), the absorption values in the spectrum of Eosin U and Azure II were 351.2 ± 17.6 and 751.4 ± 37.6 counts, respectively. For animals with BLV and BIV mono-infection, these indicators were 253.3 ± 12.7; 383.3 ± 19.2 and 371.5 ± 18.5; 500.2 ± 24.9 counts. While in intact cows, these indicators were registered at the level of 210.3 ± 10.5 and 173.6 ± 8.6 counts. In intact animals, the acidic and basic components ratio in the cell was uniformly proportional, the ratio coefficient was 0.83 ± 0.04. For BIV, BLV and BLV/BIV-infected animals, this coefficient was 1.34 ± 0.06, 1.51 ± 0.08 and 2.13 ± 0.11, that was in 1.6, 1.8 and 2.6 times higher than in intact animals, which might be an indicator of metabolic acidosis in the cell.