Preventive potency of sumbawa forest honey on rats exposed by lead acetate based on liver histopathology and AST-ALT level

Abstract

Abstract Many cattle are kept in near garbage dump and eat waste product. The cattle will exposure free radical of lead. Sumbawa forest honey contains flavonoids as antioxidant that neutralize the excess free radicals in the body. This research aimed to examine the preventive potency of Sumbawa forest honey toward liver histopathology and AST-ALT level on rats induced by lead acetate. The subject research were rats Wistar, male and age 8-12 weeks. The rats divided in 5 different groups, those were negative control group; positive control group that exposed by lead acetate; and preventive therapy groups with Sumbawa forest honey dose of 25 mg/kgBW, 50 mg/kgBW and 75 mg/kgBW for 28 days and administered with lead acetate dose of 10 mg/day for 14 days. AST-ALT levels were measured by spectrophotometer then analyzed statistically by One Way ANOVA and Tukey test (p<0.05). Liver histopathology was examined descriptively on HE stained. The result showed the forest honey Sumbawa dose 75 mg/kg BW was able to prevent ALT-AST elevation significantly (p<0.05) and reduced the injury of liver tissue on rats exposed lead. The conclusion was the Sumbawa forest honey can be used as a preventative to inhibit the liver injury because of Lead exposure.