Genetic evaluation of Central Javanese local duck based on the ovalbumin gene

Abstract

Abstract Duck farm industry has a big contribution to the supply of animal protein sources in Indonesia. Reproductive efficiency in poultry is determined by the factor of the high ratio of hatching and fertilization, fertile duration, egg weight, and the number of eggs. Reproductive efficiency control genes play a major role in parent selection and provision of superior duck seeds. This study aims to evaluate the genetic quality of local ducks in Central Java based on the ovalbumin gene using PCR-RFLP technique. A total of 35 central Javanese local ducks were feathered on the inside of the right and left wings. Duck DNA is isolated from a feather sample. The ovalbumin gene was amplified with PCR techniques using specific primers TovaF1 and TovaR1. The product of the ovalbumin gene amplification (350 bp) is subsequently cut with restriction enzyme SspI (5′-AATATT-3′). DNA bands from restriction enzyme cutting then analyzed its genotype. The results showed that 6(17.14%) ducks had CC genotype, 16(45.71%) with TT genotype, and 13(37.14%) of CT genotype. Ducks with CC and TT genotypes showed higher hatchability compared to CT genotype. It was concluded that 22(62.86%) central Javanese local ducks were genetically superior in terms of hatchability (genotype CC and TT).