Evaluation of Total Phenolic, Total Flavonoid, and In Vitro Cytotoxic Activity of Syzygium cumini Extract in Cervical Cancer Cell

Abstract

Abstract Side effect of conventional cancer terapy has driven researches to find alternative therapy. People in the province of West Nusa Tenggara, especially the Sasak tribe, perform medical treatments that refer to the traditional Lontar Usada manuscript. One of the plants mentioned in this manuscript was jamblang plant (Syzygium cumini). This study aims to explore and determine the potential of S. cumini leaves extract as an anti-cervical cancer. Extraction was carried out by the Soxhlet method using ethyl acetate, methanol, and water as solvents. The extract obtained was tested for Thin Layer Chromatography (TLC), FTIR test, and cytotoxicity test for the MTT method using HeLa cells. TLC exhibited that the extract contain phenol and flavonoid. FTIR analyzed that the extract had functional groups O-H phenols, C-H alkanes, C=C alkenes, C=0 ketone, C=C aromatic ring, NO2 nitro compound, and C=C alkenes. Total phenolic content and total flavonoid content of ethyl acetate, methanolic and water extract were 443.80 + 0.33; 305.80 + 0.28; and 45.80 + 0.11 mg GAE / g, and 74 + 0.12; 70 + 0.28; and 34 + 0.21 mg QE / g. Ethyl acetate extract showed highest cytotoxicity with IC50 value 330.50 + 1.59, followed by methanol extract 378.35 + 2.84 and water extract 3608.84 + 0.85.