Relationship between of Insuline Like growth Factor-2 Gene and Growth Traits in Crosses of Indonesian Local Chicken

Abstract

Abstract Kedu chicken is known as a laying hen, while Sentul and Kampung chicken are known as dual-purpose type chickens. The crossing of Sentul, Kedu and Kampung chicken were produced SKKedu and KeduSK chickens. One of gene that control growth is IGF-2 (Insuline-like Growth Factor 2). This study aims to analyze the diversity of IGF-2 genes and analyze association of genes with growth in SKKedu and KeduSK chickens. The samples used were 23 KeduSK chicken DNA and 17 SKKedu chicken DNA 12 weeks old. Growth measurements consist of body weight, body weight gain, feed consumption and feed conversion ratio. DNA extracted from the blood samples were analysed by PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). The diversity of IGF-2 genes was analysed with X2 test. The results showed of IGF-2 gene in SKKedu and KeduSK chickens (CC, TC, dan TT). IGF-2 gene was polymorphic and was in a Hardy-Weinberg equilibrium state. The growth traits of SKKedu and KeduSK chickens based on differences in genotype on IGF-2 genes are almost the same,there were no differences in the growth traits. There was no correlation between IGF-2 genotype of SKKedu and KeduSK chicken with growth traits.