Synthesis of a fluorescent probe for rhodamine B and determination of Zn2+ content in pharmaceutical zinc gluconate

Abstract

Abstract The intermediate LBXJ was prepared by Rhodamine B, hydrazine hydrate and HCl, and then the probe Y2ST was prepared by LBXJ, chromone 3-formaldehyde and absolute ethanol. NMR characterized the structures of the intermediate and probe, IR and MS. Compared the fluorescence selectivity of ions after the system was determined and tested the response time, pH value, cation interference, reversibility, linearity, detection limit, accuracy, coordination ratio, and recovery rate of the samples. The detection limit of Y2ST was 0.7×10-6 mol/L, and the detection rate of Zn2+ in zinc gluconate was 96.1% at 5×10-6 mol/L--5×10-5 mol/L. It can be used for the content determination of Zn2+.