Optimization of Ultrasonic-Assisted Aqueous Two-Phase Extraction of Flavonoids from Hawthorn Leaves Using Response Surface Methodology

Abstract

Abstract In this paper, using hawthorn leaves as raw materials, the effect of extracting total flavonoids from hawthorn leaves by aqueous two-phase system (ATPS) combined with ultrasonic assisted was studied. Through single factor test and response surface method Box-Behnken design (response surface method, BBD), according to the regression equation, the optimal process conditions were obtained as follows: the ratio of solid to liquid 1:37, ultrasonic time 40 min, ultrasonic power 360 w, ultrasonic temperature 65°C, and the yield of flavonoids was 2.869±0.0004%. This study shows that ultrasound combined with aqueous two-phase system (ATPS) is an effective method to extract total flavonoids from hawthorn leaves.