New Paper-based Microfluidic Analytical Devices for Kojic Acid Assay Based on Double-sided Adhesive Tape

Abstract

Abstract Kojic acid is an antioxidant used by the cosmetics industry but the excessive usage of kojic acid may cause harm to human health. Therefore, it is important to detect the content of kojic acid in cosmetic products. In this work, the low-cost and simple paper-based analytical devices (μPADs) based on double-sided adhesive tape were developed for the colorimetric detection of kojic acid. The μPADs were fabricated by dissolving double-sided adhesive tape in toluene and then making the patterned adhesive tape penetrate into the filer paper. Ferric nitrate was used as the reagent to react with kojic acid, and the product of their chelating reaction caused the color change from colorless to orange-red. The ImageJ software was used to analyze the color intensity and the corresponding correction curve was determined. The μPADs for the detection of kojic acid exhibited a wide linear range (0.05 - 4 mM) and the detectable limit was 0.05 mM. The good selectivity and reproducibility were also demonstrated.