Isolation and characterization of bacteria from mangrove sediment at coastal area in Pangkep South Sulawesi

Abstract

Abstract Mangrove ecosystem is an area with high potential of natural resourceswhich nowadaysremains unexplored. One of them is microorganismsthathidden in the depth of the mangrove sediments. Therefore, this study aims to isolate and characterize mangrove’s sediment bacteria from estuary coast of Pangkajene River, South Sulawesi, Indonesia. Sampling activities conducted at 6 stations with 3 different depths, those are 0-15 cm, 15-30 cm and 30-45 cm. Microbiological analysis includes microscopic, biochemical and analysis of the abundance of bacterial colonies using Total Plate Count test.From isolation and characterization, it was obtained 35 bacterial isolates consist of 7 different genus, those are Bacillus, Staphylococcus, Vibrio, Macrococcus, Alteromonas, Escherichia, and Listeria. Furthermore, result fromTotal Plate Counttest obtained variedwith the highest abundance of bacterial colonies was found atthe depth of 30-45 cmwith 9.48 x 104CFU.