Bioactivity of methanolic extract of marine tunicate Pyura sp against methicillin resistant Staphylococcus aureus (MRSA)

Abstract

Abstract The research on potential methanol extract from marine tunicate Pyura sp as antibacterial Methicillin-Resistant Staphylococcus aureus (MRSA) has been done from February to April 2017. This research was intended to know the potential of methanol tunicates extract of proposed tunicates as an antibacterial MRSA. Tunicates were collected from Barrang Lompo waters of Sangkarang Archipelago of South Sulawesi Indonesia. Samples were dried, then the active compound was extracted used methanol solvent according to a standard procedure elsewhere. Tunicate’s extract was prepared into three different concentrations: 10%, 15%, and 20%, were tested against MRSA bacteria. A test of inhibition zone was applied at 24 h and 48 h incubation time in order to determine the bioactivity of tunicate’s extract towards MRSA. The result indicates that crude extracts of tunicates P. Pyura sp at all concentration performs anti bacteria activity against MRSA. This study concluded that tunicate Pyura sp has bactericidal activity against MRSA and this can lead to developing a new antibiotic in the future.