Author:
Ambarwati N S S,Elya B,Desmiaty Y,Ahmad I
Abstract
Abstract
The tyrosinase enzyme catalyzes the first stage in two reactions in the synthesis of melanin (the hydroxylation of l-tyrosinase becomes 3,4-dihydroxyphenylalanine (l- DOPA) and oxidation from L-dopa to dopaquinone). Hyperpigmentation in human skin is a general phenomenon that is not desirable. Researchers were encouraged to identify potential new tyrosinase inhibitors for cosmetics, especially anti-hyperpigmentation. This study purposed to determine the inhibitory activity of the tyrosinase enzyme from Garcinia daedalanthera Pierre leaves. The extract was obtained by maceration successively method. The anti-tyrosinase assay used the spectrophotometric method at 490 nm. The enzyme used tyrosinase from mushrooms lyophilized powder (Sigma), and the substrate used 3,4-dihydroxy-L-phenylalanine, L-DOPA (Sigma). The tyrosinase inhibitory assay results of 100 ppm (triplicate) showed the leaves extract, including ethyl acetate extract of 33.42 ± 5.98 %, hexane extract was 50.67 ± 0.47 %, and methanol extract of 50.68 ± 1.87 %, respectively. Moreover, the stem bark has activity as follows methanol extract of 43.76 ± 1.41 % and the ethyl acetate extract of 55.71 ± 2.80 %. Percentage inhibition of the positive control (kojic acid) was 65.07 ± 0.03 % at 100 ppm.
Subject
General Physics and Astronomy
Reference13 articles.
1. Tyrosinase Inhibitory Activity of Flavonoids from;Nguyen;Artocarpus heterophyllous Chem. Cent. J.,2016
2. Antioxidant and Tyrosinase Inhibitory Activities of Seed Oils from Torreya grandis Fort. ex Lindl;Cui;Hindawi BioMed Res. Int.,2018
3. A Comprehensive Review on Tyrosinase Inhibitors;Zolghadri;J. Enzyme Inhib. Med. Chem.,2019
4. Pharmacognostical and Phytochemical Evaluation Leaves Extract of Garcinia daedalanthera Pierre;Najib;J Young Pharm,2017
5. Evaluation of Antimicrobial Activities of Garcinia latissima Miq. Stem Bark Extract;Ambarwati;J. Young Pharm.,2017
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献