The performance of survival and immunity of catfish (Clarias gariepinus) juvenile infected by Aeromonas hydrophila after treated by prebiotic extract in the diet

Abstract

Abstract Herb/plant can act as a prebiotic. Red ginger has long known as an anti-inflamation and anti-oxidant. Similarly, moringa leaf is also known as herbal antibiotic. In the recent years, the use of natural ingredients to stimulate immune system and to combat fish disease in aquaculture becoming a favourable choice. The aims of this research was to observe the red ginger and moringa leaf extractin the diet to the performance of catfish infected by Aeromonas hydrophila. Experimental laboratory using a completely randomized design with 6 treatments and 3 replication used in this study. The treatments were mixture of (A) 500 ppm and 0 ppm, (B) 375 ppm and 150 ppm, (C) 250 ppm and 300 ppm, (D) 125 ppm and 450 ppm, (E) 0 ppm and 600 ppm, (F) 0 ppm and 0 ppm mixture of red ginger and moringa leaf extract respectively. One hundred and eighty catfish juvenile were reared in 18 aquarium. They were fed with various concentration of prebiotic mixture for 14 days. They were infected with 1 mL A. hydrophila at 1.2 105cell/cc intramuscularly and observed for 10 days for their survival and immune response. Results showed that addition of red ginger and moringa leaf extract significantly improve the immunity of catfish. The addition of moringa leaf extract at 600 ppm was significantly improved survival rate, phagocytosis activities, leucocyte, lymphocytes count compared to other prebiotic mixtures. Thus, the usage of 600 ppm moringa leaf extract in the diet provided the best performance to catfish.