Research on dynamic perfusion control technology of 3D printed tissue vascular network

Abstract

Abstract In order to solve the problem that the medium cannot be supplied accurately during the perfusion culture of the vascular network in large-volume 3D printing tissue, a perfusion control technology was studied by designing and building an in vitro perfusion and monitoring platform with feedback regulation. First, a large-volume vascular network of hydrogel material was fabricated by 3D bioprinting technology as the experimental object of perfusion. Then we designed and built the dynamic perfusion device and the monitoring equipment in the perfusion process independently and applied the real-time flow calibration control technology on this basis. Finally, the accuracy of the flow real-time calibration control technology was verified through the perfusion experiment of the large-volume vascular network. The results showed that the error rate between the actual flow rate and the preset flow rate increased gradually with the decrease of the preset flow rate of medium perfusion. When the preset flow is 3 mL/min, the error rate between them is as high as 19.33% without the application of flow calibration control technology. The dynamic perfusion device can reduce the average error rate between the two from 8.1% to 0.4% after applying real-time flow calibration control technology, which proves that the device can cultivate a large-volume vascular network with high-precision perfusion after the application of real-time flow calibration control technology.