Study of Phytochemical, Antibacterial Activity and Toxicity on Acetone Extract Seed LeersiaHexandraSw

Abstract

Abstract The Sayat-sayat(LeersiahexandraSw) plant is a plant used in traditional medicine by the Batak Karo people in North Sumatra. This study aims to examine the phytochemical content, antibacterial activity and cytotoxic test of acetone extract of L.hexandraSw seeds. Phytochemical tests include tests for alkaloids, flavonoids, saponins, tannins and steroids. Antibacterial tests were carried out against Propionibacterium acnesATCC (27853), Escherichia coli ATCC 25922, Bacillus cereus ATCC 1178, Salmonella enterica ATCC 14028 with the paper disc diffusion method M02-A11 (CLSI) and continued with the determination of MIC and MBC using the microdilution method M07-A9 (CLSI). The toxicity test used the Brint Shrimp Lethality Toxicity (BSLT) method using Artemia Salina Leach shrimp larvae. The results of phytochemical screening showed that the acetone extract of the L.hexandraSw seeds contained alkaloids, flavonoids and terpenoids. The results of the antibacterial activity test showed the best activity against B. cereus ATCC 1178 and S. enterica ATCC 14028 with an inhibition zone in the range of 8.4-8.6mm, with MIC and MBC values of 625 μg/mL. The results of the toxicity test are toxic and potentially bioactive.