Determination some virulence genes (avrxacE2, avrxacEl, hrpG, acrAB) in Pantoea spp. isolated from hospitals of Al-Diwaniyah city, Iraq

Abstract

Abstract Pantoea spp. is one of the opportunistic pathogens, which causes the spread of hospital infection due to its transformation from bacteria to plant organisms to human pathogenic bacteria. A total of 623 samples were collected and distributed as 483 samples of different clinical cases (77.52%) and 140 environmental samples (22.47%) from Diwaniyah city hospitals. The results showed that 24 isolates of pantoea spp.s had been confirmed using API-20E Polymerase chain reaction technology. The current study reported the presence of two types of pathogens for the Pantoea spp.: the first is Pantoea agglomerans and Pantoea calida in hospitals in the city of Diwaniyah. Pantoea agglomerans was occurring in the studied isolates at a higher rate (2.27%) than Pantoea calida. All isolates of Pantoea spp were tested for the prevalence of virulence genes (avrxacE2, avrxacEl, hrpG, acrAB) using the polymerase chain reaction (PCR) technique. The highest incidence of hrpG was recorded as 79.16%, followed by avraxacE2 gene were 13 (54.16%). The current study did not record any presence of the virulence genes (avrxacE1 acrAB) among the isolates of Pantoea spp.