Colorimetric Determination of Phenylephrine hydrochloride Drug by Diazotization Reaction

Abstract

Abstract By diazotization coupling reaction is one of the most frequently employed technique in pharmaceutical analysis. It involves measuring the amount of ultraviolet or visible radiation absorbed by a substance in solution as a method for estimation drug of Phenylephrine hydrochloride by reliant on azo-coupling reaction between (PHE)HCl and Clonazepam as a reagent. To reach to the aim of obtaining the colored product with a maximum absorbance 466nm. The linearity range for Phenylephrine hydrochloride was (0.5_9μg\ml) while the detections limit 0.006 and quantification limit 0.02μg\ml The Molar Absorptivity 2.5457 ×104L/mol.cm with Sandal’s sensitivity 0.01μg\cm2. Eventually these methods were successfully completed to determination of PHE.HCl in pure and commercial form.