Derivative Uv-Spectroscopic determination of theophylline, salbutamol sulfate and glycerylguaicolate in syrup mixture

Abstract

Abstract This study aimed to determine theophylline, salbutamol sulfate and glycerylguaiacolate using ultraviolet spectrophotometry derivative. Research conducted in syrup was analyzed using zero crossing wavelength method. NaOH 0,1 N was selected as the solvent. Zero crossing wavelength of theophylline was 275 nm on the first derivative, salbutamol sulfate was 262 nm on the second derivative and glycerylguaiacolate was 243 nm on the third derivative. Results showed that the recovery of theophylline and glycerylguaiacolate were between 112,913%-118,353% and 116,129%-117,655%, respectively, while the relative standard deviation was between 1,090%-1,903% for theophylline and 1,013%-1,922% for glycerylguaiacolate. Limits of detection of theophylline and glycerylguaiacolate were 1,626 ppm and 6,375 ppm, respectively while limits of quantification were 4,927 ppm and 19,319 ppm. This results Thus, it was concluded that this method could be applied to determine theophylline and glycerylguaiacolate in syrup mixture.