Design and Optimization of Herbal Formulations in Silver Nanoparticles to Enhance Wound Healing Activity

Abstract

Abstract Wound healing is a biological process that involves a series of consecutive process, and its impairment can lead to chronic wounds and various complications. Recently, there has been a growing interest in employing nanotechnology to enhance wound healing. Silver nanoparticles (AgNPs) have expanded significant attention due to their wide range of applications in the medical field. The advantages of AgNPs include their easy synthesis, change their shape, and high surface area. Silver nanoparticles are very efficient for topical drug administration and wound healing because of their high ratio of surface area to volume. A distinguishing sign of a blessure is the disruption of the epidermal integrity of the skin. Generally speaking, the body’s natural defense against injury is wound healing. Bruised wounds may result in more difficult-to-treat, chronic wounds. Here the use of ethanolic extract of S. alternata and A. indica, silver-nanoparticles were obtained for the research. It has been tested if these nanoparticles are effective in both laboratory conditions and animals. The characterization of nanoparticles plays an important character, and it is accomplished through the use of several characterization methods such as UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). Both ethanol distillate products include bioactive secondary metabolites according to traditional phytochemical analyses and GC-MS. Two Methanol extracts were used in the metal reduction method to obtain biosynthesize silver nanoparticles. Testing the in-vitro cytotoxic and wound closure properties of methanol silver nano-particlesis done. To create transdermal patches, silver methanol nanoparticles from S. alternata and A. Indica were used. Transdermal patches made of silver ethanol nanoparticles were used in an in vivo investigation to help treat wounds. Sitosterol, cyclobarbital, 4-ethylbenzoic acid, antra-9,10-quinone, 2-p-nitrophenyl-oxadiazol-1,3,4-one-5, and butylparaben are secondary bioactives of S. Alternata ethanol, according to a phytochemical analysis. The ethanolextract from A. indica also demonstrates that both methanol-based silver nanoparticles with L6 cells have excellent migration properties after 24 hours. In the F2 formulation, both ethanolic silver NP transdermal patches offer excellent drug leasing properties. Live research on wound healing has shown that the F2 formulation improves wound healing compared to conventional medications. The size of the animal wounds completely decreased after 14 days in comparison to the usual medication dosage. As a result, transdermal patches based on silver-nanoparticles in methanol are unquestionably a great way to treat wounds. The suggested formulation aids in improving wound therapy by including many bioactive phytocomponents.