The effect of black cumin (Nigella sativa) extract on spermatozoa morphology of male albino rats (Rattus norvegicus) after induced with 2-Methoxyethanol

Abstract

Abstract The purpose of this study was to determine the effect of 2-ME and administration of black cumin extract on sperm motility of male white rats. The research method used a completely randomized design (CRD) of 24 male white rats. The 2-ME solution is given intraperitoneally, and black cumin extract is given orally. The samples were divided into 6 groups, treatment given for 21 days. Data analysis used One Way ANOVA and continued with Duncan’s test. The results, after induced 2-ME spermatozoa morphology mean as much as 31.25%, after being given average black cumin extract spermatozoa morphology of 89.75%, after administration of 2-ME and black cumin extract dose of 1.2 g/kgBW average morphology of spermatozoa 35.75%, the dose of 2.4 g/kgBW mean morphology of spermatozoa was 47.00%, while the dose of 3.6 g/kgBW resulted in an increase in morphology of spermatozoa by 57.25%. Conclusion, 2-ME induction can reduce the morphology of male white rat spermatozoa. While the administration of black cumin extract can improve the morphology of male white rat spermatozoa. The administration of black cumin extract at a dose of 3.6 g/kgBW after 2-ME induced can improve the morphology of the sperm and is significantly different.