An extract of protein pili and filtrat of human spermatozoa: A chemical review

Abstract

Abstract This study aims to determine the effect of E. coli pili protein, filtrate media culture of E. coli and mixture of E. coli pili protein and E. coli filtrate on the motility and vitality of human sperm in an in vitro. The method for obtaining E. coli pili protein is E. coli prepared by do culture on TCG media to grow E. coli pili, then cutting the Pili E. coli using Omni mixer. Pili that has been obtained then dialysis, electrophoresis and electroelution. E. coli filtrate was obtained by the method of culturing E. coli on Mc Conkey media for 48 hours and then doing culture on BHI media for 48 hours. E. coli culture on BHI media was then centrifuged. E. coli cultures in BHI media were centrifuged and the supernatant produced from centrifugation was filtered using a. 0.22µm milipore filter. 10 samples of normal donor sperm according to 1999 WHO criteria, in preparation using Percoll method. Spermatozoa were incubated with E. coli pili protein, E. coli filtrate and a mixture of E. coli pili proteins and E. coli filtrate on U-base microplate. Incubation was performed for 1 hour. Observations by calculating the motility and vitality of spermatozoa each at 100 spermatozoa under a light microscope. Data analysis was done with SPSS. The results of this study found that Protein Pili E. coli, E. coli filtrate and a mixture of E. coli pili proteins and E. coli filtrate had significant effect on sperm motility (p = 0.000) and on sperm vitality (p = 0.000). Thus it can be concluded that E. coli pili protein and E. coli filtrate are potentially used for spermicidal materials.