The potency of B-G31 isolate associating with valanganigricornis as a probiotic candidate to digest cellulose

Abstract

Abstract Cellulose is a polymer that is abundant in the environment, but they are unable to digest by the human digestive system. This study aims to determine the anti-pathogenic ability and measure the cellulase activity of B-G31 isolate. Auto-aggregation and co-aggregation methods were used to analyze the anti-pathogenic role of B-G31 against biofilm formed by Escherichia coli and Staphylococcus aureus. To assess glucose concentration obtained from cellulose degradation, B-G31 supernatant was reacted in different CMC concentrations (0.5%, 1%, 1.5%, 2%, and 2.5%) and was measured their absorbance (OD540) using ELISA spectrophotometer. The study has revealed that the percentage of B-G31 auto-aggregation is 26% and they can explicitly inhibit colonization of E. coli and S. aureus biofilm accounted for 20.21% and 21.20%, respectively, the bacteria also exhibited antagonistic activity towards two bacterial pathogens. Furthermore, enzyme activity was relatively high in the presence of 2% CMC with 0.913 U/mL to yield average glucose of 411.75 ppm and significantly different from that in the control group (p < 0.05). However, the enzyme value in 0.5% CMC concentration was about 0.345 U/mL and not significantly different from control group (p > 0.05). Our results indicated that B-G31 isolated from Valanganigricornis can form aggregates against bacterial-tests biofilm and increase degradation of cellulose, thus, the isolate could probably be used as probiotics to digest cellulose.