Valve-based consecutive bioprinting method for multimaterial tissue-like constructs with controllable interfaces

Author:

Wang HeranORCID,Guo Kai,Zhang Liming,Zhu Huixuan,Li Shijie,Li Song,Gao Feiyang,Liu Xin,Gu QiORCID,Liu LianqingORCID,Zheng XiongfeiORCID

Abstract

Abstract Bioprinting is a promising technology focusing on tissue manufacturing, whose vital problem is the precise assembly of multiple materials. As the primary solution, the extrusion-based multi-printhead bioprinting (MPB) method requires printhead switching during the printing process, which induces inefficient motion time and material interface defects. We present a valve-based consecutive bioprinting (VCB) method to resolve these problems, containing a precise integrated switching printhead and a well-matched voxelated digital model. The rotary valve built-in the VCB printhead guarantees the precise assembling of different materials at the interface isolated from the viscoelastic inks’ elastic potential energy in the cartridge. We study the coordinated control approach of the valve rotation and pressure adjustment to achieve the seamless switching, leading to a controllable multimaterial interface, including boundary and suture structure. Furthermore, we compare the VCB method and MPB method, quantitatively and comprehensively, indicating that the VCB method obtained greater mechanical strength (maximum tensile deformation increased by 44.37%) and higher printing efficiency (effective time ratio increased by 29.48%). As an exemplar, we fabricate a muscle-like tissue with a vascular tree, suture interface encapsulating C2C12, and human dermal fibroblasts (HDFB) cells, then placed it in complete medium with continuous perfusion for 5 d. Our study suggests that the VCB method is sufficient to fabricate heterogeneous tissues with complex multimaterial interfaces.

Funder

The National Key Research and Development Program of China

the Strategic Priority Research Program of the Chinese Academy of Sciences

Fundation of State Key Laboratory of Robotics

the National Natural Science Foundation of China

the Research Equipment Development Program of the Chinese Academy of Sciences

the National High Technology Research and Development Program of China

Publisher

IOP Publishing

Subject

Biomedical Engineering,General Medicine,Biomaterials,Biochemistry,Bioengineering,Biotechnology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3